Consecutive staining with Romanowsky and periodic acid Schiff reagents.
نویسندگان
چکیده
منابع مشابه
Detecting glycogen in peripheral blood mononuclear cells with periodic acid schiff staining.
Periodic acid Schiff (PAS) staining is an immunohistochemical technique used on muscle biopsies and as a diagnostic tool for blood samples. Polysaccharides such as glycogen, glycoproteins, and glycolipids stain bright magenta making it easy to enumerate positive and negative cells within the tissue. In muscle cells PAS staining is used to determine the glycogen content in different types of mus...
متن کاملModified technique for periodic acid-Schiff staining of glycoproteins on agarose-film electrophoretograms.
We have modified the periodic acid-Schiff staining technique for glycoproteins for use with thin agarose-film electrophoresis membranes. With this procedure, carbohydrate-rich proteins can be detected with negligible background staining and insignificant staining of nonglycoproteins such as albumin and nonglycosylated Bence Jones proteins (free light chains). On the other hand, carbohydrate-ric...
متن کاملThe reliability of periodic acid-Schiff staining in the diagnosis of onychomycosis.
Sir, We read with great interest the article on Comparison of potassium hydroxide mount and mycological culture with histopathological examination using periodic acid-Schiff staining of nail clippings in a recent issue of IJDVL.[1] The authors findings about high sensitivity of periodic acid-Schiff (PAS) staining in the diagnosis of onychomycosis is consistent with previous studies on the su...
متن کاملStaining properties and stability of a standardised Romanowsky stain.
An evaluation of the standardised Romanowsky stain of Marshall et al. has been made in a routine haematology laboratory. It was noted that this stain had several advantages over the May-Grünwald Giemsa stain used in most British laboratories. These advantages include ease and speed of preparation, a shorter staining time, and reproducibility of results. These results are described in detail. Th...
متن کاملA new staining method using a hematoxylin, alcian blue and periodic acid-Schiff reaction for demonstrating mucins in gastric glands.
Mucous neck cells in the rat stomach which contain neutral mucin were stained with a modified Mayer's hemalum. When alcian blue staining and periodic acid-Schiff (PAS) reaction preceded hematoxylin staining, mucin-containing fundic gland cells could be distinguished in the same tissue sections: surface mucous cells were positive for PAS reaction and/or alcian blue staining, neutral mucin-contai...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1986
ISSN: 0021-9746
DOI: 10.1136/jcp.39.12.1364-b